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KMID : 0350519920450010207
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Journal of Catholic Medical College
1992 Volume.45 No. 1 p.207 ~ p.214
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Comparison among Agglutination, Immobilization and Immunobead Test Detecting Antisperm Antibodies in Sera of Unexplained Infertile Women
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Abstract
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There are several methods of detecting antisperm antibodies in serum including method of sperm agglutination(Franklin-Duke test; F-D test), sperm immobilization(SIT), antiglobulin uptake, and immunofluorescences.
Recently, much attention has been directed to the immunobead binding test(IBT). It allows us to determine the degree of binding and even to locate antibody bindng site on the sperm itself.
This test detect sperm-bound antibodies by using polyacrylamide bead which is coated with rabbit anti-human IgG, IgA, IgM respectively.
This study was designed to compare the new IBT methodology with the classical tests, F-D test and SIT.
Sera from 89 unexplained infertile wemen were assayed for antisperm antibodies using the F-D test, SIT and IBT simultaneously.
Also, we tried to determine the immunoglobulin isotype of antisperm antibodies and its binding site on sperm.
@ES The results were as follows;
@EN 1. Among 89 infertile women, IBT-positive was found in 18 cases(20.2%) and IBT-negative was 71 cases(79.8%), and 15 cases(16.9%) were positive for SIT, compared to 20 cases(22.5%) for F-D test. Of the 18 IBT positive, 13 cases(72.2%) were
positive
for SIT and 7 cases(38.9%) were F-D positive.
In the 71 IBT negative group, however, it is found that the SIT positive was 2.9% (2 out of 71), and F-D positive was 18.3%(13 out of 71) respectively. The correlations between IBT and SIT was staustically significant(P<0.001) in the detection
rate
of
sperm antibodies, while those between IBT and F-D test showed no such statistical significance(P>0.05)(Table 1, 2).
2. The immunoglobulin of 18 IBT-positive sera was found to be IgG in 14 cases(77.7%), IgA in 11 cases(61.1%), and IgA in 4 cases(22.2%). The 13 cases was positive for both IBT and SIT. Among them, IgG was detected in 10 cases(76.9%), IgA in 8
cases(61.5%), and IgM in 3 cases(23.1%). Likewise, 7 cases showed positive in both IBT and F-D test.
In this group, 6 cases(85.7%) have IgG, 4 cases(57.1%) have IgA , 1 case(14.3%) has IgM respectively(Table 3, 4).
3. In IBT positive infertile women, the binding site of antisperm antibodies was directed to the head in 64.3% of IgG, tail in 63.7% of IgA, and tail tip in 75% of IgA respectively(Table 3, 4).
The significant correlation were noted between the SIT and IBT, Finally made it possible to conclude that the SIT can replace IBT as a antisperm antibody detection method in a clinical setting. Most of antisperm antibodis in sera of unexplained
infertile women, another our major concern, consists of IgG and IgA which located in the sperm head and at the tail respectively.
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